ProtumLink Union 25cm

The 25 cm column is developed for researchers requiring higher resolving power to confidently analyze complex or fractionated samples.

At 400 nL/min, FWHM values of ~4.2–5.5 s across 30–60 min gradients offer the extended separation necessary to reduce co-elution and co-isolation, which directly enhances identification depth and quantification quality. This format is particularly effective for biomarker discovery and deep profiling applications.

Operating within 100–800 nL/min, the 25 cm column is optimized for nanoflow high-resolution separations on UHPLC systems. It provides deeper biological insight without requiring excessively long runs, making it suitable for advanced discovery and complex experimental designs.

Union columns feature an integrated liquid junction that enables straightforward connection to nano-LC systems using standard 10-32 threaded fittings. This configuration provides a robust, zero-dead-volume interface and allows fast column exchange without additional adapters. The integrated junction can also serve as the grounding or high-voltage application point when required by the instrument configuration. Union columns are fully compatible with setups using Thermo Scientific Nanospray Flex ion sources, where the high voltage can be applied through the liquid connection. This standardized connectivity enables a direct and reliable link between the column outlet and the UHPLC system while preserving excellent chromatographic performance. Specific adapter configurations may be required depending on the vendor ion source when operating emitter-integrated columns with a pre-mounted liquid junction. Users should consult instrument documentation where compatibility with blank-emitter columns is explicitly described.

• Thermo Scientific nanoViper™ Fingertight Fittings • IDEX Health & Science MarvelXACT™ UHPLC Connection Systems • Waters ZenFit™ Connection Technology (compatibility pending verification)