Integrated precision for every LC–MS workflow
Flexible designs that deliver stable, high-performance nanoflow separations
Product Highlights
Robustness
Sensitivity
Next-generation integrated emitter technology delivers exceptional robustness, extended column lifetime, and enhanced analytical sensitivity.
Effortless
Integration
Versatile adapter compatibility enables plug-and-play usability across established LC–MS workflows, ensuring seamless workflow integration.
Performance
Accessibility
Delivering high-end performance at accessible pricing, providing reliable chromatographic solutions for laboratories of all scales.
Configurable
Selection
Available in lengths from 5 – 50 cm and inner diameters of 75 or 150 µm, with custom configurations available upon request.
Robustness
of column lifetime
ProtumLink's column architecture combines the mechanical durability of traditional emitter-separate setups with the sensitivity and spray stability of integrated emitter designs.
A reinforced packing bed and precisely shaped emitter tip maintain uniform flow and stable electrospray, supporting consistent performance over extended use. This integrated design reduces mechanical stress and minimizes signal fluctuations, resulting in longer column lifetimes and sustained analytical sensitivity, even in demanding high-throughput environments.
4 adapter types
Core
Blank Capillary Columns
Union
With Liquid Junctions (<1200bar)
Connect
With Liquid Junctions (<1200bar) and CSI-Adapter
Extend
Column cassette (< 1200bar) compatible with EASY-Spray™ Source
5 column lengths
5cm
8cm
15cm
25cm
50cm
Doesn't fit your needs?
Performance
of robust column set up
High-resolution peak separation is maintained across the different column lengths, demonstrating robust chromatographic performance of ProtumLink's 75 µm ID designs. The presented FWHM values show narrow peak widths under commonly used injection amounts and gradient durations. This illustrates the expected separation efficiency for the respective setups and supports reliable identification depth and consistent quantification in routine proteomics.
Testimonials
Dr. Hannes C. A. Drexler
Head Mass Spectrometry Unit, MPI für Molecular Biomedicine, Münster
For many years, we were highly confident in the quality of our self-packed capillary columns. However, we eventually had to acknowledge that the ProtumLink Core columns clearly outperform our own columns packed with 1.5 µm C18 beads in terms of robustness, reproducibility and the number of protein IDs obtained. We therefore decided to discontinue packing our own capillary columns, an easy decision given how reasonably priced the ProtumLink columns are.
Dr. Stefan Walter
Head Mass Spectrometry Core Facility, University of Osnabrück
We use ProtumLink Connect columns in our core facility because we are impressed by their performance, reproducibility, and long service life. Based on this experience, it was an obvious decision to use these columns exclusively on a larger scale, given their balanced performance-to-price ratio.
Dr. Alfredo Cabrera-Orefice
AG Cabrera - Proteomics Core Facility, Institute of Biochemistry, Justus-Liebig University, Giessen
The columns from ProtumLink we evaluated demonstrated excellent performance, robustness and long-term stability. They were tested for global proteome profiling as well as complexome profiling, enabling the analysis of large sample series over extended periods (weeks to months) without significant changes in electrospraying and backpressure. These columns are highly user-friendly, flexible and truly plug-and-play, while delivering consistent and reproducible results. The products are well suited as a robust and one-stop solution for routine and high-throughput proteomics workflows.
Application examples
Routine quality control measurements of 50 ng HeLa on a 25 cm × 75 µm Connect column with a 60 min gradient demonstrate highly consistent proteome depth across more than 700 LC-MS injections on a timsTOF HT system.
Retention time coefficients of variation for precursor ions, assessed on randomly selected QC runs throughout the column lifetime, confirm excellent chromatographic stability, which is essential for reliable label-free quantification.
In cooperation with Dr. Stefan Walter, Mass Spectrometry Core Facility, University of Osnabrück.
Application Notes
Ultra robust performance stability of 25 cm × 75 µm Emitter-Integrated Nanoflow Column with 1.9 µm C18 Material over 800 Measurements
PDF - 1.5 MB
High-Sensitivity and Reproducible DIA Performance of a 15 cm × 75 µm Emitter-Integrated Nanoflow Column on the Orbitrap Astral System
PDF - 1.1 MB
FAQs
Column Adapter Types
Core
Emitter-integrated analytical columns designed for maximum flexibility in system configuration and custom workflows. Ideal for method development and broad instrument compatibility.
Union
Emitter-integrated analytical columns featuring an integrated liquid junction for easy connection to standard nano-LC fittings.
Connect
Emitter-integrated analytical columns equipped with a Bruker CaptiveSpray adapter for native compatibility and true plug-and-play operation on timsTOF platforms.
Extend
Emitter-integrated analytical columns in a Thermo EasySpray compatible format using a pre-mounted emitter cassette design for simplified installation and reliable performance.
Core
Emitter-integrated analytical columns designed for maximum flexibility in system configuration and custom workflows. Ideal for method development and broad instrument compatibility.
Union
Emitter-integrated analytical columns featuring an integrated liquid junction for easy connection to standard nano-LC fittings.
Connect
Emitter-integrated analytical columns equipped with a Bruker CaptiveSpray adapter for native compatibility and true plug-and-play operation on timsTOF platforms.
Extend
Emitter-integrated analytical columns in a Thermo EasySpray compatible format using a pre-mounted emitter cassette design for simplified installation and reliable performance.
The Lab And Me
