ProtumLink Union 8cm

The 8 cm format provides additional resolving power over shorter columns while still supporting the speed needed for routine quantitative proteomics.

Measured at 800 nL/min, the column delivers consistent peak widths of ~3.4–4.2 s across 10–20 min gradients, offering focused peak geometry that improves duty-cycle efficiency and quantification reliability. This makes the format well suited for time-sensitive cohort studies and routine translational workflows.

An operational flow window of 100–1500 nL/min allows smooth movement between accelerated profiling and nanoflow-level sensitivity without system changes. The 8 cm length provides a practical balance of speed, resolving power, and robustness, making it suitable for laboratories running a broad mix of LC-MS assays.

Union columns feature an integrated liquid junction that enables straightforward connection to nano-LC systems using standard 10-32 threaded fittings. This configuration provides a robust, zero-dead-volume interface and allows fast column exchange without additional adapters.

The integrated junction can also serve as the grounding or high-voltage application point when required by the instrument configuration. Union columns are fully compatible with setups using Thermo Scientific Nanospray Flex ion sources, where the high voltage can be applied through the liquid connection.

This standardized connectivity enables a direct and reliable link between the column outlet and the UHPLC system while preserving excellent chromatographic performance.

Specific adapter configurations may be required depending on the vendor ion source when operating emitter-integrated columns with a pre-mounted liquid junction. Users should consult instrument documentation where compatibility with blank-emitter columns is explicitly described.

• Thermo Scientific nanoViper Fingertight Fittings • IDEX Health & Science MarvelXACT UHPLC Connection Systems • Waters ZenFit Connection Technology (compatibility pending verification)