ProtumLink Union 5cm

The ProtumLink 5 cm emitter-integrated column is designed for laboratories prioritizing high sampling throughput while maintaining the sensitivity needed for limited-input material. It is a reliable choice for rapid discovery and scouting experiments.

Even under accelerated conditions, the 5 cm format maintains sharp and stable peak profiles. At 1 µL/min, FWHM values remain consistently in the 3.1–4.1 s range across 5–15 min gradients, supporting confident peptide quantification and robust detection in high-volume workflows and QC environments.

With a usable flow range from 100 nL/min (nanoflow) up to 2000 nL/min (capillary flow), the column adapts well to different analytical needs. Its low backpressure enables operation at up to 1 µL/min even on pressure-limited systems, making it easy to transition between rapid screens, QC checks, and sensitive low-input analyses without changing hardware.

Union columns feature an integrated liquid junction that enables straightforward connection to nano-LC systems using standard 10-32 threaded fittings. This configuration provides a robust, zero-dead-volume interface and allows fast column exchange without additional adapters.

The integrated junction can also serve as the grounding or high-voltage application point when required by the instrument configuration. Union columns are fully compatible with setups using Thermo Scientific Nanospray Flex ion sources, where the high voltage can be applied through the liquid connection.

This standardized connectivity enables a direct and reliable link between the column outlet and the UHPLC system while preserving excellent chromatographic performance.

Specific adapter configurations may be required depending on the vendor ion source when operating emitter-integrated columns with a pre-mounted liquid junction. Users should consult instrument documentation where compatibility with blank-emitter columns is explicitly described.

• Thermo Scientific nanoViper Fingertight Fittings • IDEX Health & Science MarvelXACT UHPLC Connection Systems • Waters ZenFit Connection Technology (compatibility pending verification)