ProtumLink Extend 25cm

The 25 cm column extends separation space beyond mid-length formats, directly increasing peak capacity and reducing co-elution across complex peptide mixtures.

At 400 nL/min, FWHM values of ~4.2–5.5 s across 30–60 min gradients provide the resolving power required for deeper identification and improved precursor separation. The increased column length enhances proteome depth without requiring extreme gradient durations.

Operating within 100–800 nL/min, the 25 cm format is optimized for high-resolution nanoflow separations where peak capacity is the primary driver of identification depth.

Extend columns are supplied in a Thermo EasySpray-compatible cassette format, enabling direct installation on EasySpray ion sources without additional emitter hardware or external high-voltage components. Electrical contact and emitter alignment are established automatically through the cassette interface.

The Extend format follows widely used heater geometries, enabling compatibility with common external column heating systems.

At the inlet side, an integrated liquid junction allows straightforward connection to nano-LC systems using standard 10-32 threaded female fittings, enabling fast column exchange while preserving zero-dead-volume performance.

This configuration supports reliable nanospray performance, easy system setup, and seamless operation on Thermo Orbitrap platforms equipped with EasySpray sources.

• Thermo Scientific nanoViper Fingertight Fittings • IDEX Health & Science MarvelXACT UHPLC Connection Systems • Waters ZenFit Connection Technology (compatibility pending verification)