ProtumLink™
Packages
Column ID (Diameter)
All prices are quoted net and are exclusive of tax and shipping fees.
Core Benefit: Increased peak capacity for complex proteomes and in-depth discovery
The 25 cm column extends separation space beyond mid-length formats, directly increasing peak capacity and reducing co-elution across complex peptide mixtures.

At 400 nL/min, FWHM values of ~4.2–5.5 s across 30–60 min gradients provide the resolving power required for deeper identification and improved precursor separation. The increased column length enhances proteome depth without requiring extreme gradient durations.
Operating within 100–800 nL/min, the 25 cm format is optimized for high-resolution nanoflow separations where peak capacity is the primary driver of identification depth.

Extend columns are supplied in a Thermo EasySpray-compatible cassette format, enabling direct installation on EasySpray ion sources without additional emitter hardware or external high-voltage components. Electrical contact and emitter alignment are established automatically through the cassette interface.
The Extend format follows widely used heater geometries, enabling compatibility with common external column heating systems.
At the inlet side, an integrated liquid junction allows straightforward connection to nano-LC systems using standard 10-32 threaded female fittings, enabling fast column exchange while preserving zero-dead-volume performance.
This configuration supports reliable nanospray performance, easy system setup, and seamless operation on Thermo Orbitrap platforms equipped with EasySpray sources.
• Thermo Scientific nanoViper Fingertight Fittings • IDEX Health & Science MarvelXACT UHPLC Connection Systems • Waters ZenFit Connection Technology (compatibility pending verification)
| Property | Specification |
|---|---|
| Silica Brand | ReproSil-Pur 120 C18-AQ (Dr. Maisch GmbH) |
| Chromatographic Mode | Reversed-phase |
| Particle Size | 1.9 µm |
| Pore Size | 120 Å |
| Surface Area | 300 m²/g |
PDF - 1.5 MB
PDF - 1.1 MB
| Bonding / Phase |
| C18 (ODS), fully end-capped, aqueous-compatible |
| Carbon Load | 15% |
| pH Stability Range | 2 – 8 |
| Max Operating Pressure | ≤ 1200 bar |
| Max Operating Temperature | 60 °C* |
*Ultra-fast gradients may be operated up to 70 °C.
We rely on a well-established C18 material from Dr. Maisch because it has been widely used in LC–MS–based proteomics for nearly two decades and is known for its stable bonding chemistry, consistent material quality, and reproducible performance. This makes it a dependable foundation for achieving high-quality peptide separations across diverse workflows.
The 1.9 µm C18 particles provide a balanced combination of peak capacity, mechanical robustness, and retention stability, supporting sharp and predictable peak shapes under the aqueous mobile-phase conditions typical for proteomics.