ProtumLink™
Packages
Column ID (Diameter)
All prices are quoted net and are exclusive of tax and shipping fees.
Core Benefit: Fast gradients with strong depth and resolving power
The 15 cm emitter-integrated column provides an optimal balance between gradient time and separation space. It enables fast to mid-length gradients while maintaining high peak capacity and confident proteome coverage.

At 500 nL/min, FWHM values of ~3.5–4.6 s across 15–45 min gradients demonstrate that separation efficiency is preserved even under accelerated conditions. This format delivers strong identification depth without extending runtimes unnecessarily, making it a versatile choice for both discovery and quantitative workflows.
Operating across 100–1000 nL/min, the 15 cm format maintains resolving performance across a wide range of gradient lengths. It represents the practical midpoint between rapid screening formats and deep-resolution columns.

Extend columns are supplied in a Thermo EasySpray-compatible cassette format, enabling direct installation on EasySpray ion sources without additional emitter hardware or external high-voltage components. Electrical contact and emitter alignment are established automatically through the cassette interface.
The Extend format follows widely used heater geometries, enabling compatibility with common external column heating systems.
At the inlet side, an integrated liquid junction allows straightforward connection to nano-LC systems using standard 10-32 threaded female fittings, enabling fast column exchange while preserving zero-dead-volume performance.
This configuration supports reliable nanospray performance, easy system setup, and seamless operation on Thermo Orbitrap platforms equipped with EasySpray sources.
• Thermo Scientific nanoViper Fingertight Fittings • IDEX Health & Science MarvelXACT UHPLC Connection Systems • Waters ZenFit Connection Technology (compatibility pending verification)
| Property | Specification |
|---|---|
| Silica Brand | ReproSil-Pur 120 C18-AQ (Dr. Maisch GmbH) |
| Chromatographic Mode | Reversed-phase |
| Particle Size | 1.9 µm |
| Pore Size | 120 Å |
| Surface Area | 300 m²/g |
PDF - 1.5 MB
PDF - 1.1 MB
| Bonding / Phase |
| C18 (ODS), fully end-capped, aqueous-compatible |
| Carbon Load | 15% |
| pH Stability Range | 2 – 8 |
| Max Operating Pressure | ≤ 1200 bar |
| Max Operating Temperature | 60 °C* |
*Ultra-fast gradients may be operated up to 70 °C.
We rely on a well-established C18 material from Dr. Maisch because it has been widely used in LC–MS–based proteomics for nearly two decades and is known for its stable bonding chemistry, consistent material quality, and reproducible performance. This makes it a dependable foundation for achieving high-quality peptide separations across diverse workflows.
The 1.9 µm C18 particles provide a balanced combination of peak capacity, mechanical robustness, and retention stability, supporting sharp and predictable peak shapes under the aqueous mobile-phase conditions typical for proteomics.