ProtumLink Core 8cm

The 8 cm format provides additional resolving power over shorter columns while still supporting the speed needed for routine quantitative proteomics.

Measured at 800 nL/min, the column delivers consistent peak widths of ~3.4–4.2 s across 10–20 min gradients, offering focused peak geometry that improves duty-cycle efficiency and quantification reliability. This makes the format well suited for time-sensitive cohort studies and routine translational workflows.

An operational flow window of 100–1500 nL/min allows smooth movement between accelerated profiling and nanoflow-level sensitivity without system changes. The 8 cm length provides a practical balance of speed, resolving power, and robustness, making it suitable for laboratories running a broad mix of LC-MS assays.

Core columns are bare fused-silica emitter-integrated analytical columns designed to be connected via a liquid junction. With an outer diameter of 363 μm, they are compatible with liquid junctions engineered for standard 360 μm fused-silica tubing. Depending on the instrument setup, metal junctions can provide grounding or high-voltage transfer, while PEEK junctions may be used when an external grounding or voltage supply point is available.

Core columns can also be mounted directly in nanospray sources that are designed to accept bare emitter tips. This includes Thermo Scientific Nanospray Flex and Bruker CaptiveSpray sources (with appropriate emitter positioning accessories such as the CaptiveSpray (CSI) emitter adjustment kit), enabling use of the column itself as the electrospray emitter and minimizing post-column volume.

Specific adapter configurations may be required depending on the vendor ion source when using bare emitter columns. Users should consult instrument documentation where compatibility with blank-emitter columns is explicitly described.

• Thermo Scientific 6041.5290 nanoViper Fingertight Fitting System, 20 μm

• IDEX UH-750 VHP MicroTee • IDEX UH-634 VHP MicroTight