Product Description
High-Speed Proteomic Screening
Core Benefit: Rapid profiling for high-throughput and screening-driven workflows
The ProtumLink 5 cm emitter-integrated column is designed for laboratories prioritizing high sampling throughput while maintaining the sensitivity needed for limited-input material. It is a reliable choice for rapid discovery and scouting experiments.
Chromatographic Excellence (FWHM Resolution)
Even under accelerated conditions, the 5 cm format maintains sharp and stable peak profiles. At 1 µL/min, FWHM values remain consistently in the 3.1–4.1 s range across 5–15 min gradients, supporting confident peptide quantification and robust detection in high-volume workflows and QC environments.
Backpressure Flexibility & Workflow Versatility
With a usable flow range from 100 nL/min (nanoflow) up to 2000 nL/min (capillary flow), the column adapts well to different analytical needs. Its low backpressure enables operation at up to 1 µL/min even on pressure-limited systems, making it easy to transition between rapid screens, QC checks, and sensitive low-input analyses without changing hardware.
Pressure profiles
Compatibilities
Core columns are bare fused-silica emitter-integrated analytical columns designed to be connected via a liquid junction. With an outer diameter of 363 μm, they are compatible with liquid junctions engineered for standard 360 μm fused-silica tubing. Depending on the instrument setup, metal junctions can provide grounding or high-voltage transfer, while PEEK junctions may be used when an external grounding or voltage supply point is available.
Core columns can also be mounted directly in nanospray sources that are designed to accept bare emitter tips. This includes Thermo Scientific Nanospray Flex and Bruker CaptiveSpray sources (with appropriate emitter positioning accessories such as the CaptiveSpray (CSI) emitter adjustment kit), enabling use of the column itself as the electrospray emitter and minimizing post-column volume.
Specific adapter configurations may be required depending on the vendor ion source when using bare emitter columns. Users should consult instrument documentation where compatibility with blank-emitter columns is explicitly described.
Compatible Connection Systems
• Thermo Scientific 6041.5290 nanoViper Fingertight Fitting System, 20 μm
Recommended Liquid Junction Options
• IDEX UH-750 VHP MicroTee • IDEX UH-634 VHP MicroTight
C18 Bead Specification
Specification Table
| Property | Specification |
|---|---|
| Silica Brand | ReproSil-Pur 120 C18-AQ (Dr. Maisch GmbH) |
| Chromatographic Mode | Reversed-phase |
| Particle Size | 1.9 µm |
| Pore Size | 120 Å |
| Surface Area | 300 m²/g |
Application Notes
Ultra robust performance stability of 25 cm × 75 µm Emitter-Integrated Nanoflow Column with 1.9 µm C18 Material over 800 Measurements
PDF - 1.5 MB
High-Sensitivity and Reproducible DIA Performance of a 15 cm × 75 µm Emitter-Integrated Nanoflow Column on the Orbitrap Astral System
PDF - 1.1 MB