ProtumLink Core 15cm

The 15 cm emitter-integrated column provides an optimal balance between gradient time and separation space. It enables fast to mid-length gradients while maintaining high peak capacity and confident proteome coverage.

At 500 nL/min, FWHM values of ~3.5–4.6 s across 15–45 min gradients demonstrate that separation efficiency is preserved even under accelerated conditions. This format delivers strong identification depth without extending runtimes unnecessarily, making it a versatile choice for both discovery and quantitative workflows.

Operating across 100–1000 nL/min, the 15 cm format maintains resolving performance across a wide range of gradient lengths. It represents the practical midpoint between rapid screening formats and deep-resolution columns.

Core columns are bare fused-silica emitter-integrated analytical columns designed to be connected via a liquid junction. With an outer diameter of 363 μm, they are compatible with liquid junctions engineered for standard 360 μm fused-silica tubing. Depending on the instrument setup, metal junctions can provide grounding or high-voltage transfer, while PEEK junctions may be used when an external grounding or voltage supply point is available.

Core columns can also be mounted directly in nanospray sources that are designed to accept bare emitter tips. This includes Thermo Scientific Nanospray Flex and Bruker CaptiveSpray sources (with appropriate emitter positioning accessories such as the CaptiveSpray (CSI) emitter adjustment kit), enabling use of the column itself as the electrospray emitter and minimizing post-column volume.

Specific adapter configurations may be required depending on the vendor ion source when using bare emitter columns. Users should consult instrument documentation where compatibility with blank-emitter columns is explicitly described.

• Thermo Scientific 6041.5290 nanoViper Fingertight Fitting System, 20 μm

• IDEX UH-750 VHP MicroTee • IDEX UH-634 VHP MicroTight