ProtumLink Connect 50cm

The 50 cm column is the most resolving option in the series, intended for the highest-depth analyses, where maximum peak capacity and sensitivity are required.

Measured at 250 nL/min, the column achieves ~5.7 s FWHM over ~95 min gradients, offering the separation space necessary to distinguish low-abundance and closely eluting peptides. This is particularly impactful for PTM mapping, deep clinical proteomics, and complex structural studies.

With an operational range of 100–400 nL/min, the 50 cm column performs reliably over long analytical sequences and requires stable UHPLC pressure capability. It is the preferred option when analytical depth, precision, and confidence take priority over throughput.

Connect columns feature a pre-installed CaptiveSpray Inlet (CSI) adapter at the emitter end, enabling direct use on Bruker CaptiveSpray and CaptiveSpray Ultra ion sources without additional emitter hardware. At the inlet side, an integrated liquid junction allows straightforward connection to nano-LC systems using standard 10-32 threaded female fittings, enabling fast column exchange and preserving zero-dead-volume performance.

In Bruker configurations, grounding is applied through the liquid junction, ensuring proper electrical contact and stable electrospray operation.

This configuration provides a robust nanospray interface with minimal post-column dispersion, stable spray performance, and seamless integration with Bruker timsTOF platforms.

• Thermo Scientific nanoViper Fingertight Fittings • IDEX Health & Science MarvelXACT UHPLC Connection Systems • Waters ZenFit Connection Technology (compatibility pending verification)